Thermoestable isomerase processes for biotechnology.

This is a three year project starting in 2017.

Thermoestable isomerase processes for biotechnology (TIPs). European Union. Horizon 2020. ERA-IB2 7th call. ERA-IB-16-049. Industrial Biotechnology for Europe. An Integrated Approach; Ministerio de Economía y Productividad, Acciones de Programación Conjunta Internacional 2016. PCIN-2016-129.

Isomers are molecules with identical atomic composition but with different structural characteristics. Different isomers can show very distinctive function. Isomerases are enzymes catalyzing the conversion between different types of isomers. Thermostable isomerases are desired because they possess high resistance and durability, are able to withstand harsh industrial process conditions, including heating and organic solvents. Elevated temperatures can also enhance substrate accessibility and solubility. The proposed project includes comparative bioinformatic analyses of sequence data to identify different classes of thermostable isomerases of industrial interest which will be cloned, over-expressed, functionally and structurally characterized and optimized towards their biotechnological application. Three types of isomerases will be targeted: sugar isomerases (to produce new desirable sugars for calorie-free sweeteners and as building blocks for drugs), disulfide isomerases (to improve protein folding and stability of industrial enzymes), and chalcone isomerases (involved in the transformation of flavonoids, secondary metabolites of importance as natural colorants, anti-oxidants, anti-microbial and anti-inflammatory agents). Durable isomerases will allow new opportunities for green, competitive and sustainable biotechnological processes that can replace conventional chemical synthesis.

Participants:


See also the ERA-IB2 link at this link.



Supported by a grant from European Union. Horizon 2020. ERA-IB2 7th call. ERA-IB-16-049. Industrial Biotechnology for Europe. An Integrated Approach.




Results

Here, you can consult some of the results and communications from this project. If you need additional information, please, contact us.



IRNAS-CSIC, Avda. Reina Mercedes 10, 41012-Sevilla, Spain
Tel. +34 95 462 4711 (ext. 146); Fax +34 95 462 4002
E-mail: jmgrau@irnase.csic.es